A tractor/trailer was involved in a highway accident, resulting in the release of approximately 75 gallons of diesel fuel from a ruptured saddle tank.  Free petroleum product flowed into the gravel road shoulder and adjacent embankment.  The site is a single lane, north/south highway of typical asphalt paved construction.  Multiple major underground communication utilities are present in the accident area making it difficult to excavate.

Accident site in WV: A network of utility lines.	Diesel Stained skirting of pavement: Indicated  damage to asphalt.

With cables significantly reducing the area of excavation accessibility and time constraints of two-way traffic closure, the decision to apply petroleum degrading microbes was made. In situ bioremediation using AgroRemed was considered a less disruptive and safe method for cleanup.  Repair technicians had not arrived at this time and AgroRemed was readily available on site. 

Laboratory results for soil samples collected after 35 days of application were as follows;  Sample-1 showed below laboratory detection limits of 50 ppm, Sample-2 showed 570 ppm, Sample-3 showed 830 ppm, and Sample-4 showed 290 ppm.  These results indicate an aggressive degradation of petroleum hydrocarbons by AgroRemed.  Additional application was made after these samples were collected.  Application of bioremediation product under such a situation was justified.  Total remediation of diesel fuel was completed in 35 days and the residual contamination did not warrent continued treatment and the state DEP closed the project.